New office employee Gary (Hader) gets into a fight to the death with incumbent cubicle holder Steve (Dane Cook). Sudeikis appears as the boss and Samberg, Forte, Armisen, Thompson, Poehler and Wiig are other employees cheering on the fight.

Harpoon Man (John C. Reilly), a suave action hero and parody of Shaft, tracks down an insulting announcer dressed as a whale (Samberg), who is narrating his life in a theme song. There are appearances by Jorma Taccone, who plays a man getting robbed, and Bill Hader, who plays the man robbing Taccone.

A fast food boss (Armisen) gives his employees (Forte, Matthew Fox, Poehler, Samberg, Thompson) a pep talk and has trouble controlling his anger until one of his employees (Forte) comes in late. It originally was scheduled to air on the episode hosted by Matthew Fox (which explains his appearance), but ended up airing on the following episode hosted by Annette Bening.

A Christmas song about two men (Justin Timberlake and Samberg) giving their lovers (Kristen Wiig and Maya Rudolph) a box with their genitalia inside as presents, in a style reminiscent of early 1990s R&B sex ballads made popular by acts such as Bell Biv Devoe, Color Me Badd, and R. Kelly. Won a 2007 Creative Arts Primetime Emmy for Outstanding Music and Lyrics. Music created in part by Asa Taccone, Jorma Taccone and Katreese Barnes. It was the first official single from The Lonely Island's debut album, Incredibad.

In the same frame-story format, Hader and Samberg apologize to Lorne Michaels for the original Laser Cats! claiming to understand where they went wrong: not enough politics. They then introduce Laser Cats! 2, based on the same premise, now set in the Iraq War which has gone nuclear and caused the feline mutations. This time, Dr. Scientist (Jake Gyllenhaal) has stolen the cure that turns Laser Cats back into regular cats. Jorma Taccone appears in the opening credits for Laser Cats! 2 (Which is the same as Laser Cats!), and Fred Armisen and Amy Poehler are the scientists who created the cure.

Scott Garbaciak (Samberg) is the multi-role star in a commercial for the fictional film Nurse Nancy, in parody of Eddie Murphy films such as Norbit and The Nutty Professor.

Drew Barrymore is Desiree, host of a 1986 sexually suggestive, low-impact, high-result exercise video Body Fuzion, with "her friends" Donna, Michelle, and Donna M. (Rudolph, Wiig, and Poehler respectively).

Samberg quietly pops into view as the camera routinely cuts to different locations and landmarks. Forte begins doing the same, before being forced out by Samberg at gunpoint. Samberg wears his "Andy" shirt from the failed sketch show Awesometown created by The Lonely Island.

A corporate executive (Rainn Wilson) leads a meeting to take ideas on how to save his failing company, and hears suggestions from an increasingly bizarre set of employees, including a gigantic turkey sub and Arcade Fire. Upon reaching the end of the meeting, Wilson receives a phone call, seemingly informing him that the office building is about to be blown up. Then, the office building explodes, killing all of them inside. Amy Poehler, Bill Hader, Jason Sudeikis, Kristen Wiig, Will Forte, Darrell Hammond, Fred Armisen, Maya Rudolph and Andy Samberg all play employees of Wilson's, and Kenan Thompson plays a water guy.

Super Bowl MVP Peyton Manning takes time out to be a mentor to kids, but ends up being physically, mentally, and verbally abusive to them (a parody of his public service announcements for the United Way during 2001–05).

In a spoof of The O.C. episode "The Dearly Beloved", a man (Hader) writes a letter to his sister, he is shot by his friend (Samberg), leading to a series of overly dramatic, slow-motion shootings set to "Hide and Seek" by Imogen Heap, including their roommate (Shia LaBeouf), the sister (Wiig), and two police officers (Sudeikis, Armisen).

Samberg performs a rhyme about how much he likes his brother in-law named Roy (SNL writer Bryan Tucker).

While meeting the owner of an apartment he's about to rent (Zach Braff), a man (Samberg) is shocked to find out that the owner's dog can not only talk (with the voice of SNL writer Jorma Taccone), but has fallen in love with him. Later he finds out that the dog was using him to get a plate of ham. The owner implies that this happens regularly, but the dog convinces the man that it's still something more, and the man ends up french kissing the dog.