A female dog from a wealthy aristocratic family named Violet Vanderfeller is being pursued by dognappers when she is picked up and taken to the city pound. There, she meets the gang and discovers their mission to find homes for themselves. The film plot centers on Violet (whom Cooler insists on referring to as "Sam") attempting to reunite with her family, ultimately succeeding. Two other dogs appeared in the special. They were the upper-class snob Barkerville and the garbage-can-digging Scrounger.

On a magical artifact called the Bone of Scone, that gives "Puppy Power" to the Pound Puppies and Pound Purries. However, a villain named Marvin McNasty plans to take it and use it for world domination. Without the Bone of Scone, humans will not understand what the animals are saying and, if it is broken, Puppy Power will forever be lost.