Journey to the Microcosmos

Every experiment has to start somewhere. This one began with a container full of dying microbes, and the five cute, pink ciliates called blepharisma that James, our master of microscopes, accidentally turned into a group of cannibals.

We’re starting this episode out with a question that we’re never going to have a good answer for: how many cells do animals have? How could we ever hope to count all those cells in each of those animals? And how could we even begin to assume that the amount of cells in one individual is going to be the same for all the other individuals?

Somewhere around 470 million years ago, something happened that shouldn’t have been particularly striking. An algae found its way onto land. This algae turned the lands of this earth green, altered the chemistry of our atmosphere, and created homes for future life. This algae would give rise to all of the land plants we know of today.

When you’re in the business of hunting for microbes, sometimes you have to send some weird emails. That’s why James, our master of microscopes, sat down one day to send his own strange request to the people at Coralaxy, a coral farm in Germany.

We’ve spent most of our journey through the microcosmos seeking out the organisms that are too small to see with just the human eye. The bacteria, the ciliates, the tardigrades. Part of what makes them so exciting to find is that they are so tiny. Every moment we spend with one of these organisms is a peek into something exceptional in our experience of the world, and it’s the result of how much work James, our master of microscopes, has put into hunting down as many microbes as he can.

James, our master of microscopes, recently received a package from a coral farm in Germany. We’ve explored some of the microscopic creatures and bristle worms that were living and thriving in those packages in previous videos. But today we’re here to focus on the main event: the corals.

We’re going to see a type of motion over and over again because it’s all over the microcosmos, found in and around many different types of organisms. And this kind of random motion may seem almost too trivial to discuss, but this motion that you see is a proof of something fundamental not just to life, but to existence itself. This movement… is proof… of atoms.

For an activity that mostly involves sitting and staring, microscopy is a surprisingly high stakes task. On the other side of the lens are drops full of potential, a multitude of worlds to unravel and examine. But they’re also fragile worlds, easy to fracture and lose with just a tiny slip of the hand. The stakes only get higher when you’re dealing with an organism so rare that it’s only been reported a few times since it was first discovered in 1901.

You’ve heard those worm horror stories, right? Stories of painful stomach cramps or diarrhea or nausea that eventually turns out to be caused by some worms that have taken up residence in someone’s intestines. It’s so terrifying and wild to think of something so much smaller than us causing so much havoc. But, what if worms had to worry about their own guts being taken over by a parasite?

This Loxodes magnus is large, so large that it was able to eat a rotifer, those funny animals we often see getting bullied by their single-celled neighbors. Except, that rotifer is moving. It’s alive, twisting and turning inside of the food vacuole it’s been stuffed into, and starting to fight back.

At first glance, they seem a bit more like plants or a series of flowers with thin, elegant petals. But no, they are indeed an animal. One that has the dubious honor of being defined largely by its anus.

James, our master of microscopes, is not a farmer. He is, to put it simply, fascinated by microbes. And that may lead him to strange places and cause him to grow tanks full of weird things. But he is not a farmer.

The theme of today's episode is pretty simple: things we never thought we’d be showing you, but here we are.

It’s often said that one person’s trash is another person’s treasure. And surely there is no greater proof of that than the home of our master of microscopes, James. All along the windowsills and bookshelves are jars and tanks full of samples gathered from ponds, lakes, and oceans. And even his cabinets and drawers and bathroom hold stockpiles of what he’s found. There is just one problem though... the snails.

From our vantage point, as relatively large organisms, it can be easy to overlook the microcosmos, because it’s simply too small to see. It floats in front of our eyes at all times, and yet we cannot make out details until we turn to other tools.

Tardigrades have been through a lot. They’ve been sent to the moon. They’ve had the moisture sapped out of them. At times, they’ve been in extreme heat. And at other times, they’ve had to contend with extreme cold. Well, today, we’ve got a new one for you. A harrowing journey for these tardigrades that have taken them through, what we assume, must be the worst thing that tardigrades have yet been subjected to. These poor, enduring tardigrades got stuck in postal security.

The ciliates we’re going to talk about today are kind of…frustrating. At this point in our journey, we’ve gotten used to the fact that the microcosmos is an indecipherable mess at times, filled with organisms that look like each other, and who have familial relationships that seem obvious but then turn out to be a figment of our own limited imaginations. And these ciliates are yet another entry in the long-standing saga of ever-changing taxonomies that define our understanding of microbial species. The plot twist is inevitable.

The microcosmos might seem like a safe place from a surprise spider attack, but it would be misleading to pretend that it’s completely free of spider-like sightings. Because even at this small scale, you could find yourself subject to an ambush of the arachnid sort.

Usually on Journey to the Microcosmos, we spend our time looking at living organisms, things like insects, plants, and microbes that move and breathe and grow and die. But today, for these first few moments, these are the only living organisms we’ll be showing you, a montage of creatures whose bodies all share one very eye-catching trait: crystals.

Depending on your love of horror stories or your belief in the supernatural, it might be easy to convince you that lakes are full of ghosts. That as you plunge deeper into these lakes’ depths, you’ll come across translucent bodies that come alive when nighttime sets in; with its limbs all packed close to the head, wrenching open and closed like scissors that propel our spectral friend in jarring motions.

When James first saw these bacteria, all he knew is that they came from a sample taken from a Portuguese beach. And on the slide, the bacteria were swimming in a stark line. And that gave James an idea. He took out his phone and opened up his compass app. Then he placed the phone on the microscope stage to see what direction the bacteria were swimming in. And he found that the bacteria were all swimming north.

Our oceans and lakes are filled with copepods, a myriad of small crustacean species that might float as plankton or infect other creatures1. And as they’re living in whatever manner best suits them, some copepods—like our friend here—become more than just their own creature. They become a surface, a place for someone else (or something else) to settle down upon.

This is kentrophoros, a ciliate that James—our master of microscopes—had been searching for, receiving samples from all over the world in the hopes of finding it gliding around. When you first look at it, it doesn’t seem particularly special. But there are two things that the kentrophoros is famous for. The first is its lack of a mouth. The second is its coat of bacteria.

As animals, we owe a lot to the single-celled organisms that came before us. These are the organisms that laid the chemical groundwork for how we live, from the DNA and proteins within them to the molecules they released into the environment. There’s something humbling about looking at our hands or feet and imagining the mixture of cells within them, and realizing the lessons that keep those cells bound together physically and biologically are rooted in a very ancient study in cooperation.

One day, James—our master of microscopes—was cleaning the marine tanks that some of his organisms live in when he noticed this creature. It was hard to miss given that it was visible to the naked eye, thanks to both its bright red color and large size.

James, our master of microscopes, gets samples of sand from beaches all over the world to help in his quest to learn more about interstitial ciliates—the single-celled organisms that live in the watery pockets that exist between grains of sand on the beach. But today, we’re going to shift our focus and let those grains be the focus of our show. More specifically, we’re going to talk about sand.

If you’ve ever wondered what it might take to upset a microscopist, just ask James—our master of microscopes—his feelings about tardigrade legs. Yes, tardigrade legs. Those chunky, wiggly limbs that move their owner through meals of moss and fields of debris. What could possibly be in question when it comes to tardigrade legs?

It’s fun to watch organisms eat in the microcosmos. There’s a whole range of methods to enjoy. And at the core of all this is a simple, universal need: energy, stored chemically as adenosine triphosphate—or ATP—that’s made from the breakdown of sugars and fats.

If you’ve been following James, our master of microscopes, on some of his other platforms, then you know what’s coming. You know that James has published his first academic paper, it's about this extraordinarily rare ciliate that you see now called Legendrea loyezae.

To study organisms at the genetic level, we need their DNA. Which means that we need to be able to wade through all the bits and pieces lying within their tiny bodies to pick out something even tinier—something we can’t just dig out with a shovel. So how does James manage to get the precious DNA from Legendrea loyezae and the other ciliates he’s interested in studying?

Sometimes our journey through the microcosmos feels like an expedition, a voyage filled with deep dives into the masses of organisms basking under the glow of our microscope. So what does it mean when you don’t find anything. When you gather your samples and excitedly prepare them for the microscope, only to find a landscape lacking in the life you expected to find?

Watching this Peranema feels a bit like watching a cat waffling back and forth between whether or not it wants to take a nap. Sometimes the Peranema stretches, its body undulating into an elongated, indescribable geometry as its flagella twitch like whiskers. And then, sometimes, it curls up into a cozy circle, tucking one end into itself the way any feline friend you might know curls up around the perfect beam of sunshine.

Imagine that this is the beginning of the last thing you’ll ever see, an empty landscape with thin lines scratched across it. But those lines suddenly sharpen and gather into a dense mass that spreads from the crown that sits atop a giant, studded with greens and yellows. A giant that is in search of one thing: food.

The Gastrotrich has long been a personal favorite microbe of several members of the Journey to the Microcosmos crew. But while we were able to see a lot with the microscopes we had at the time, James—our master of microscopes—has made some significant upgrades since then and this means that we are now able to see gastrotrichs in a whole new light.

Science is built on questions. So let’s start today with one: what do you think happens when you set off an electrical spark in the microcosmos?

If you’ve been with us on our journey for a while, you’ve probably heard us say the phrase “we don’t know” a lot. The microcosmos doesn’t guarantee answers, and we’ve often found ourselves looking at some unusual behavior or beautiful form that represents some fascinating, unresolved mystery.

You may not want to think about it this way, but your mouth is really just one giant, wet cave for microbes. From the perspective of bacteria, your mouth is not a tool. It is a home. It is a place that provides shelter and food, but it is also a place that can pose many threats. And the interplay between our mouths and the microbes that take up residence within them ends up, inevitably, affecting our own health.

This might not look like much. But every day, tiny little things like this are raining down on our planet. Each one is small, about a millimeter across. But over the course of a year, each individual piece that makes its way to Earth’s surface adds up to around 30,000 tons.

The microcosmos is not always a graceful space. Sometimes an organism just needs to get around the way it gets around, even if that means looking like a swimming elephant head with a truncated snout at one end and a rat tail at the other.